Composite

Part:BBa_K4773016:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-02)


Lac Promoter+RBS+ptb+RBS+buk+RBS+adhE2+RBS+ATF1+Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1838
    Illegal BglII site found at 1979
    Illegal BglII site found at 2759
    Illegal BglII site found at 4840
    Illegal BglII site found at 6117
    Illegal XhoI site found at 5960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 6544
    Illegal SapI.rc site found at 7552


Design Notes

Different restriction sites were added between each part to facilitate the connection of each part or further manipulation.Term (BBa_B0015) was chosen because it is a double terminator, which can effectively terminate transcription and protect mRNA from degradation.


Source

ptb: From the genomic sequence of Clostridium acetobutylicum. buk: From the genomic sequence of Clostridium acetobutylicum. ATF1: From the genomic sequence of Saccharomyces cerevisiae.

References